3EL2
Crystal Structure of Monomeric Actin Bound to Ca-ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-09-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 206.634, 54.023, 36.972 |
| Unit cell angles | 90.00, 98.16, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.231 |
| Rwork | 0.203 |
| R-free | 0.26200 |
| Starting model (for MR) | Protein only from 3EKU |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.093 | 0.268 |
| Number of reflections | 13523 | |
| <I/σ(I)> | 12.8 | 3.2 |
| Completeness [%] | 95.8 | 77.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 277 | 20% MPD, 0.1M sodium acetate, 0.1M NaCl, 0.02M CaCl2, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
