3EJY
Structure of E203H mutant of E.coli Cl-/H+ antiporter, CLC-ec1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Detector technology | CCD |
| Collection date | 2008-01-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.919 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.516, 96.830, 172.790 |
| Unit cell angles | 90.00, 132.81, 90.00 |
Refinement procedure
| Resolution | 58.520 - 3.200 |
| R-factor | 0.24618 |
| Rwork | 0.244 |
| R-free | 0.27929 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ots |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 127.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.087 | 0.584 |
| Number of reflections | 46752 | |
| <I/σ(I)> | 19.6 | 3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 295 | 37 % PEG 300 (w/v), 20 mM NaBr, 50 mM glycine-NaOH, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
