3EJO
Coproporphyrinogen III oxidase from Leishmania donovani
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-26 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.067, 53.991, 65.565 |
| Unit cell angles | 86.63, 77.77, 61.25 |
Refinement procedure
| Resolution | 28.410 - 2.300 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.26700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2qt8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.068 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.5.0047) |
| Refinement software | REFMAC (refmac_5.5.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.075 | 0.224 |
| Number of reflections | 26482 | |
| <I/σ(I)> | 9.149 | 2.86 |
| Completeness [%] | 95.6 | 93.7 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.95 | 278 | 0.1 M potassium acetate (not pH'ed), 0.1 M MES (pH 5.95), 17.9% PEG 4K, 5 mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
