3EJC

Full length Receptor Binding Protein from Lactococcal phage TP901-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-28
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	H 3 2
Unit cell lengths	41.710, 41.710, 465.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.540 - 1.850
R-factor	0.19673
Rwork	0.192
R-free	0.23844
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f0c
RMSD bond length	0.013
RMSD bond angle	1.303
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.103	0.410
Number of reflections	14190
<I/σ(I)>	5.6	1.7
Completeness [%]	99.9	99.9
Redundancy	5.5	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	300 nL of protein at 5 mg/mL were mixed with 100 nL of 20% PEG 8000, 0.2 M Mg Acetate tetrahydrate, 0.1 M Na Cacodylate pH 6.5 using a Cartesian Pixsys Robot, VAPOR DIFFUSION, SITTING DROP, temperature 293K