3EJ6
Neurospora Crassa Catalase-3 Crystal Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 131.835, 154.507, 162.415 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.670 - 2.300 |
| R-factor | 0.237 |
| Rwork | 0.193 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sy7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.763 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.680 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.083 | 0.360 |
| Number of reflections | 152747 | |
| <I/σ(I)> | 7.2 | 1.7 |
| Completeness [%] | 93.5 | 85.8 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 291 | 18% PEG 4000, 50MM TRIS, pH 8.00, VAPOR DIFFUSION, temperature 291K |
