3EIX
Crystal structure of selenomethionine labelled Staphylococcus aureus lipoprotein, HtsA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97607 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.952, 43.645, 76.040 |
| Unit cell angles | 90.00, 100.57, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.350 |
| R-factor | 0.13122 |
| Rwork | 0.129 |
| R-free | 0.16579 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.402 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.11) |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 2.910 | 1.350 |
| Rmerge | 0.050 | 0.036 | 0.166 |
| Number of reflections | 62441 | ||
| <I/σ(I)> | 37.924 | ||
| Completeness [%] | 97.9 | 96.5 | 87.3 |
| Redundancy | 3.4 | 3.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.1 Hepes pH 7.0, Jeffamine ED-2001, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
