3EIV
Crystal Structure of Single-stranded DNA-binding protein from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 101.454, 104.788, 163.306 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.182 - 2.141 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.25900 |
| Structure solution method | MR, MR |
| Starting model (for MR) | 1x3e |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.915 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.200 | 2.220 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.038 | 0.350 |
| Total number of observations | 22946 | |
| Number of reflections | 31640 | |
| <I/σ(I)> | 13.373 | 1.5 |
| Completeness [%] | 65.7 | 100 |
| Redundancy | 2.44 | 2.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 50 mM TRIS-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
