3EIT
the 2.6 angstrom crystal structure of CHBP, the Cif Homologue from Burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-03 |
| Detector | RIGAKU |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.362, 78.003, 115.037 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.150 - 2.560 |
| R-factor | 0.21 |
| Rwork | 0.203 |
| R-free | 0.26700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.110 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.625 |
| High resolution limit [Å] | 2.560 | 2.559 |
| Rmerge | 0.074 | 0.278 |
| Number of reflections | 16433 | |
| <I/σ(I)> | 26.9 | 6.4 |
| Completeness [%] | 100.0 | 75 |
| Redundancy | 7.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 32% PEG1000, 100mM Sodium Cacodylate, 5% Glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
