3EIJ

Crystal structure of Pdcd4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9798
Spacegroup name	P 31 2 1
Unit cell lengths	170.325, 170.325, 66.919
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.23531
Rwork	0.235
R-free	0.27666
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.806
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SnB
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Rmerge	0.084
Number of reflections	27711
<I/σ(I)>	5
Completeness [%]	100.0
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5		20% PEG MME 2000, 0.1M Tris-HCl pH8.5, 0.2M trimethylamine N-oxide (TMAO), 10mM DTT, 10mM sarcosine, VAPOR DIFFUSION, HANGING DROP