3EIC
X-ray structure of Acanthamoeba ployphaga mimivirus nucleoside diphosphate kinase complexed with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-07-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.064 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.472, 152.726, 184.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.18997 |
| Rwork | 0.188 |
| R-free | 0.22437 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b8q |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.396 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.400 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 49557 | |
| <I/σ(I)> | 6 | 2.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 273 | MPD 40 to 44%, MOPS 0.1M, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
