3EIA

Crystal structure of K270Q variant of LL-diaminopimelate aminotransferase from Arabidopsis thaliana complexed with L-Glu: External aldimine form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-29
Detector	ADSC QUANTUM 315
Wavelength(s)	1.115872
Spacegroup name	P 32 2 1
Unit cell lengths	102.676, 102.676, 172.014
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.500 - 1.850
R-factor	0.18773
Rwork	0.186
R-free	0.22959
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z20
RMSD bond length	0.022
RMSD bond angle	1.853
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.920
High resolution limit [Å]	1.850	1.850
Number of reflections	86778
Completeness [%]	95.9	95.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	45% (NH4)2SO4, 0.1 M HEPES pH 7.5, 3% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 298K