3EH3
Structure of the reduced form of cytochrome ba3 oxidase from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | Q315R |
| Collection date | 2008-01-04 |
| Detector | ADSC Quantum-315R CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 119.750, 119.750, 150.814 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 3.100 |
| R-factor | 0.20612 |
| Rwork | 0.201 |
| R-free | 0.29991 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xme |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.768 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.030 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.075 | 0.786 |
| Number of reflections | 22612 | |
| <I/σ(I)> | 5.7 | 0.9 |
| Completeness [%] | 96.0 | 96 |
| Redundancy | 2.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | Peg 2000, Bis-tris, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
