3EH1
Crystal structure of the human COPII-coat protein Sec24b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2007-05-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.370, 67.500, 72.440 |
| Unit cell angles | 90.00, 100.37, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| Rwork | 0.184 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.280 |
| Number of reflections | 65527 | |
| <I/σ(I)> | 22.5 | 3.3 |
| Completeness [%] | 97.8 | 85 |
| Redundancy | 3.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | Protein was concentrated to 40 mg/ml in 150 mM NaCl, 20 mM Tris pH 7.5, 4 mM DTT. Crystals were grown via the hanging-drop method, by adding 1ul protein solution to 1ul of well solution comprising 42% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
