3EGQ

Crystal structure of a tetr-family transcriptional regulator (af_1817) from archaeoglobus fulgidus at 2.55 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162, 0.97927, 0.97913
Spacegroup name	P 21 21 21
Unit cell lengths	57.550, 61.040, 109.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.412 - 2.550
R-factor	0.244
Rwork	0.243
R-free	0.27500
Structure solution method	MAD
RMSD bond length	0.027
RMSD bond angle	2.262
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.412	29.412	2.630
High resolution limit [Å]	2.550	5.440	2.540
Rmerge	0.036	0.025	0.301
Number of reflections	13034	2393	2087
<I/σ(I)>	13.52	27.8	2.7
Completeness [%]	96.7	94.6	85.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.35	293	0.2M sodium chloride, 38.4% polyethylene glycol 200, 0.1M phosphate-citrate pH 4.35, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 293K