3EGM
Structural basis of iron transport gating in Helicobacter pylori ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 4 |
| Unit cell lengths | 128.528, 128.528, 165.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.950 - 2.100 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.20400 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.948 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.131 | 0.409 |
| Number of reflections | 78025 | |
| <I/σ(I)> | 22.333 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 100mM HEPES, 10% isopropanol, 200mM Sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
