3EGE
Crystal structure of Putative methyltransferase from antibiotic biosynthesis pathway (YP_324569.1) from ANABAENA VARIABILIS ATCC 29413 at 2.40 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-11 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97915 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 76.609, 76.609, 134.815 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.761 - 2.400 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.20200 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.689 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.761 | 29.760 | 2.460 |
High resolution limit [Å] | 2.400 | 10.730 | 2.400 |
Rmerge | 0.109 | 0.044 | 0.714 |
Total number of observations | 1788 | 7507 | |
Number of reflections | 18494 | ||
<I/σ(I)> | 6.022 | 12 | 1.1 |
Completeness [%] | 99.8 | 95.2 | 98.7 |
Redundancy | 8.7 | 7.4 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 277 | 31.0% ethylene glycol, 0.1M phosphate-citrate pH 4.2, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |