3EGB
Structure of Pellino2 FHA domain at 3.3 Angstroms resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-10 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.676, 89.484, 125.557 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.000 - 3.250 |
R-factor | 0.242 |
Rwork | 0.238 |
R-free | 0.31500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ega |
RMSD bond length | 0.010 |
RMSD bond angle | 1.329 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 3.380 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.160 | 0.460 |
Number of reflections | 8337 | |
<I/σ(I)> | 19 | 4.5 |
Completeness [%] | 99.9 | 99 |
Redundancy | 8.4 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 295 | 0.05 M sodium citrate, 23% w/v PEG 3350 MME, 1 M NaCl, pH 5.5, vapor diffusion, temperature 295K |