3EG4
Crystal structure of 2,3,4,5-Tetrahydropyridine-2-carboxylate N-Succinyltransferase from Brucella melitensis biovar abortus 2308
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9774 |
| Spacegroup name | H 3 |
| Unit cell lengths | 67.808, 67.808, 154.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.800 - 1.870 |
| R-factor | 0.132 |
| Rwork | 0.130 |
| R-free | 0.16600 |
| Structure solution method | MR, MR |
| Starting model (for MR) | 3bxy |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.683 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.800 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.097 | 0.442 |
| Number of reflections | 21653 | |
| <I/σ(I)> | 12.2 | 2 |
| Completeness [%] | 98.6 | 91.3 |
| Redundancy | 3.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | HAMPTON INDEX B11: 2.1M DL MALIC ACID, PH 7.0, VAPOR DIFFUSION, TEMPERATURE 298K, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
