3EFF

The Crystal Structure of Full-Length KcsA in its Closed Conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Detector technology	CCD
Collection date	2007-10-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	115.799, 173.905, 339.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.800
R-factor	0.28
Rwork	0.270
R-free	0.33100
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.900
High resolution limit [Å]	3.800	3.800
Rmerge	0.108	0.850
Number of reflections	32412
Completeness [%]	94.8	89.7
Redundancy		5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	298	340 mM (NH4)2SO4, 11% PEG4000, 100 mM Na3C6H5O7 pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K