3EFB
Crystal Structure of Probable sor Operon Regulator from Shigella flexneri
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-18 |
Detector | SBC-3 |
Wavelength(s) | 0.9793, 0.9795 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 69.536, 238.463, 53.759 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.378 - 2.001 |
R-factor | 0.203 |
Rwork | 0.200 |
R-free | 0.24900 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.164 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine)/refmac5.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.380 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.057 | 0.386 |
Number of reflections | 61178 | 1528 |
<I/σ(I)> | 6.6 | 2 |
Completeness [%] | 97.3 | 49.9 |
Redundancy | 8.7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.2M calsium acetate, 9% PEG 8000, 0.1M sodium cacodylate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |