3EF0

The Structure of Fcp1, an essential RNA polymerase II CTD phosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	55.169, 80.682, 89.342
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.100
Rwork	0.220
R-free	0.25200
Starting model (for MR)	Fcp1-BeF3-Mg model
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.069	0.239
Number of reflections	23771
<I/σ(I)>	25.159	5
Completeness [%]	99.1	98.1
Redundancy	5.9	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	Fcp1-AlF4--Mg was obtained by incubating 250 M Fcp1(149-580)- 330-393 with 500 M AlCl3, 5 mM NaF and 5 mM MgCl2 on ice for 2 h prior to crystallization by hanging drop vapor diffusion against 20% PEG-3350, 190 mM ammonium formate (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 291K