3EED
Crystal structure of human protein kinase CK2 regulatory subunit (CK2beta; mutant 1-193)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 116.463, 116.463, 76.088 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.120 - 2.800 |
| R-factor | 0.17619 |
| Rwork | 0.172 |
| R-free | 0.21616 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JWH (CHAINS C and D) |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.123 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.740 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.099 | |
| Number of reflections | 13382 | |
| <I/σ(I)> | 29.9 | 4.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 293 | 25.5% PEG 8000, 15% glycerol, 0.17M ammonium sulfate, 0.085M sodium cacodylate, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
