3EEA
The crystal structure of the GAF domain/HD domain protein from Geobacter sulfurreducens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.453, 79.471, 112.632 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.960 - 1.800 |
| R-factor | 0.20183 |
| Rwork | 0.200 |
| R-free | 0.24131 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.701 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.960 | 1.847 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.112 | 0.519 |
| Number of reflections | 28282 | |
| <I/σ(I)> | 24.57 | 1.12 |
| Completeness [%] | 98.1 | 90.48 |
| Redundancy | 6.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M CaCl2, 0.1M Tris, 20%(w/v)PEG4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
