3EDT

Crystal structure of the mutated S328N hKLC2 TPR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Wavelength(s)	1.0809
Spacegroup name	P 31
Unit cell lengths	101.202, 101.202, 115.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.700
R-factor	0.23157
Rwork	0.230
R-free	0.26691
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.943
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.700	2.700
Number of reflections	32746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	291	2.0 M ammonium Sulfate, 20% Ethylene Glycol, 0.1 M Bis-Tris propane, pH 7.0, EVAPORATION, temperature 291K
1	EVAPORATION	7	291	2.0 M ammonium Sulfate, 20% Ethylene Glycol, 0.1 M Bis-Tris propane, pH 7.0, EVAPORATION, temperature 291K
1	EVAPORATION	7	291	2.0 M ammonium Sulfate, 20% Ethylene Glycol, 0.1 M Bis-Tris propane, pH 7.0, EVAPORATION, temperature 291K
1	EVAPORATION	7	291	2.0 M ammonium Sulfate, 20% Ethylene Glycol, 0.1 M Bis-Tris propane, pH 7.0, EVAPORATION, temperature 291K
1	EVAPORATION	7	291	2.0 M ammonium Sulfate, 20% Ethylene Glycol, 0.1 M Bis-Tris propane, pH 7.0, EVAPORATION, temperature 291K