3EDO

Crystal structure of Flavoprotein in Complex with FMN (YP_193882.1) from Lactobacillus acidophilus NCFM at 1.20 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-14
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	44.560, 52.030, 156.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.424 - 1.200
R-factor	0.137
Rwork	0.136
R-free	0.16000
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.422
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.424	29.424	1.240
High resolution limit [Å]	1.200	2.580	1.200
Rmerge	0.067	0.050	0.459
Number of reflections	114258	21483	18335
<I/σ(I)>	7.6	16.1	1.9
Completeness [%]	95.9	96.6	88.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	0.2000M NH4H2PO3, 20.0000% PEG-3350, No Buffer pH 4.6, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K