3EDN
Crystal structure of the Bacillus anthracis phenazine biosynthesis protein, PhzF family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2008-08-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.089, 65.638, 150.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.180 - 1.500 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21900 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.195 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.180 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.087 | 0.445 |
| Number of reflections | 93368 | |
| <I/σ(I)> | 13 | 2.4 |
| Completeness [%] | 97.8 | 89.6 |
| Redundancy | 5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 2M NH4SO4, 5% isopropanol, pH 7.0, VAPOR DIFFUSION, temperature 293K |
| 1 | VAPOR DIFFUSION | 7 | 293 | 2M NH4SO4, 5% isopropanol, pH 7.0, VAPOR DIFFUSION, temperature 293K |
