3ECU
Crystal structure of human apo Cu,Zn Superoxide Dismutase (SOD1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-30 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54056 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.169, 33.528, 113.982 |
| Unit cell angles | 90.00, 111.64, 90.00 |
Refinement procedure
| Resolution | 39.010 - 1.900 |
| R-factor | 0.24308 |
| Rwork | 0.239 |
| R-free | 0.27909 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hl5 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.412 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.153 | 0.409 |
| Number of reflections | 42832 | |
| <I/σ(I)> | 4.4 | 4.1 |
| Completeness [%] | 97.6 | 92.9 |
| Redundancy | 6.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289.1 | 0.1 M MES 20% PEG 3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289.1K |
