3ECI
Microtubule-associated protein 1 light chain 3 alpha isoform A (MAP1ALC3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 42 |
| Unit cell lengths | 80.066, 80.066, 37.089 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.310 - 2.650 |
| R-factor | 0.2371 |
| Rwork | 0.233 |
| R-free | 0.30379 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ugm |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.348 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 7058 | |
| <I/σ(I)> | 2.19 | |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 32 % PEG 4000, 0.1 M NA ACETATE, PH 4.50, 0.2 M AMMONIUM ACETATE, VAPOR DIFFUSION, HANGING DROP, CRYOPROTECTION 20% GLYCEROL, TEMPERATURE 298K |
