3ECF

Crystal structure of an ntf2-like protein (ava_4193) from anabaena variabilis atcc 29413 at 1.90 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-06
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97864
Spacegroup name	P 41 21 2
Unit cell lengths	74.056, 74.056, 189.378
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.323 - 1.900
R-factor	0.184
Rwork	0.182
R-free	0.22200
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.683
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.323	29.330	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.154	0.053	0.668
Total number of observations		3377	22422
Number of reflections	42557
<I/σ(I)>	4.213	7.6	1.1
Completeness [%]	100.0	97.6	100
Redundancy	7.2	5.8	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	2.4000M (NH4)2SO4, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K