3EC8
The crystal structure of the RA domain of FLJ10324 (RADIL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.00849, 0.91841, 0.97968 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 46.080, 46.080, 135.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 11.630 - 2.600 |
| R-factor | 0.23149 |
| Rwork | 0.227 |
| R-free | 0.27009 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.107 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.740 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 9816 |
| Completeness [%] | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.4M Ammonium dihydrogen phosphate, 0.1M TRIS pH 8.5, 35 %(v/v) MPD , VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
