3EBN
A Special Dimerization of SARS-CoV Main Protease C-Terminal Domain Due to Domain-swapping
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-12-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.395, 51.350, 51.390 |
| Unit cell angles | 112.22, 112.00, 104.36 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.209 |
| Rwork | 0.208 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uk2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.322 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.051 | 0.190 |
| Number of reflections | 14727 | |
| Completeness [%] | 95.0 | 88.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 25% PEG3350, pH5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
