3EBE

Crystal structure of xenopus laevis replication initiation factor MCM10 internal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	110
Detector technology	CCD
Collection date	2007-03-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	54.565, 94.414, 69.775
Unit cell angles	90.00, 112.79, 90.00

Refinement procedure

Resolution	44.400 - 2.300
R-factor	0.2046
Rwork	0.202
R-free	0.24710
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.572
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	29404
<I/σ(I)>	27.7	2.3
Completeness [%]	99.6	97
Redundancy	6.7	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	PEG 4000, TRIS, KSCN, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	8	294	PEG 4000, TRIS, KSCN, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	8	294	PEG 4000, TRIS, KSCN, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 294K