3EB7
Crystal Structure of Insecticidal Delta-Endotoxin Cry8Ea1 from Bacillus Thuringiensis at 2.2 Angstroms Resolution
Replaces: 2QKGExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 407.282, 47.956, 103.121 |
| Unit cell angles | 90.00, 91.12, 90.00 |
Refinement procedure
| Resolution | 43.200 - 2.300 |
| R-factor | 0.16913 |
| Rwork | 0.162 |
| R-free | 0.23009 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ciy |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.575 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 204.100 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.137 | 0.460 |
| Number of reflections | 98915 | |
| <I/σ(I)> | 12.3 | 2.07 |
| Completeness [%] | 97.4 | 84.1 |
| Redundancy | 4.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
