3EAS

Novel dimerization motif in the DEAD box RNA helicase Hera: form 1, complete dimer, asymmetric

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2008-05-31
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	46.120, 70.770, 181.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.300 - 2.800
R-factor	0.2317
Rwork	0.228
R-free	0.31370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eaq
RMSD bond length	0.006
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.100	2.880
High resolution limit [Å]	2.800	2.800
Number of reflections	15368
<I/σ(I)>	10.1	1.4
Completeness [%]	99.9	100
Redundancy	6.3	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1