3EAD
Crystal structure of CALX-CBD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.90682 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.372, 73.722, 129.713 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.590 - 2.250 |
| R-factor | 0.21279 |
| Rwork | 0.210 |
| R-free | 0.26933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dpk |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.955 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.072 | 0.544 |
| Number of reflections | 26203 | |
| <I/σ(I)> | 3.1 | |
| Completeness [%] | 94.4 | 71.8 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 50 mM MES, 20% PEG 3350, pH 6.0, VAPOR DIFFUSION, temperature 291K |
