3EAC
Crystal structure of SH2 domain of Human Csk (carboxyl-terminal src kinase), Oxidized form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 80 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-26 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.114, 48.025, 49.866 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.360 - 1.370 |
| R-factor | 0.20934 |
| Rwork | 0.209 |
| R-free | 0.21686 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k9a sh2 part |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.370 | 1.370 |
| Number of reflections | 22360 | |
| Completeness [%] | 99.8 | |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.3 | 298 | 22% PEG 4000, 100 mM Bis-Tris, pH 7.3., EVAPORATION, temperature 298K |
