3EAA

Structure of a type six secretion system protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2007-10-30
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 6
Unit cell lengths	85.459, 85.459, 93.066
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.963 - 2.794
R-factor	0.241
Rwork	0.238
R-free	0.28400
Starting model (for MR)	PDB code 1Y12
RMSD bond length	0.007
RMSD bond angle	1.032
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.794
Number of reflections	9073
<I/σ(I)>	7.5
Completeness [%]	94.0
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.2M Ammonium sulphate, 0.1M BIS-TRIS(pH 6.5), 25% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K