3EA0
Crystal Structure of ParA Family ATPase from Chlorobium tepidum TLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.572, 67.032, 134.786 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.450 - 2.200 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.25160 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.145 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.450 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.138 | 0.658 |
| Number of reflections | 44390 | |
| <I/σ(I)> | 6.8 | 2.8 |
| Completeness [%] | 99.3 | 95.2 |
| Redundancy | 9.8 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.2M MgCl2, 0.1M BisTris pH6.5, 25 % PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
