3E98
CRYSTAL STRUCTURE OF a GAF domain containing protein that belongs to Pfam DUF484 family (PA5279) FROM PSEUDOMONAS AERUGINOSA AT 2.43 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-13 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97975 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.841, 88.114, 60.963 |
| Unit cell angles | 90.00, 107.92, 90.00 |
Refinement procedure
| Resolution | 29.412 - 2.430 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.27000 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.202 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.412 | 2.490 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.074 | 0.689 |
| Number of reflections | 20453 | |
| <I/σ(I)> | 7.038 | 1.1 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.2000M Ca(OAc)2, 10.0000% PEG-8000, 0.1M Imidazole pH 8.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, VAPOR DIFFUSION, SITTING DROP |
