3E8O
CRYSTAL STRUCTURE OF A PUTATIVE ANTIBIOTIC BIOSYNTHESIS MONOOXYGENASE (DR_2100) FROM DEINOCOCCUS RADIODURANS AT 1.40 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97916,0.97849 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.715, 59.639, 47.511 |
| Unit cell angles | 90.00, 97.18, 90.00 |
Refinement procedure
| Resolution | 29.814 - 1.400 |
| R-factor | 0.13 |
| Rwork | 0.128 |
| R-free | 0.16300 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.626 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.814 |
| High resolution limit [Å] | 1.400 |
| Rmerge | 0.050 |
| Number of reflections | 35346 |
| <I/σ(I)> | 9.2 |
| Completeness [%] | 96.3 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2000M NaOAc, 30.0000% PEG-8000, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, VAPOR DIFFUSION, SITTING DROP |
