3E8K

Crystal structure of HK97 Prohead II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	295
Detector technology	CCD
Collection date	2008-02-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9
Spacegroup name	I 2 2 2
Unit cell lengths	553.033, 574.390, 587.358
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 3.650
R-factor	0.365
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Prohead II 12A Cryo-EM model and PII 5.2A crystal structure
RMSD bond length	0.007
RMSD bond angle	1.490
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	RAVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	3.740
High resolution limit [Å]	3.650	3.650
Rmerge	0.184	0.431
Number of reflections	656590
<I/σ(I)>	2.5	1.4
Completeness [%]		14.8
Redundancy	1	1.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	295	100mM CHES pH 9.0, 200mM Manganese Chloride, 2.7% PEG 4k, 200mM NDSB-211(in drop only), VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	9	295	100mM CHES pH 9.0, 200mM Manganese Chloride, 2.7% PEG 4k, 200mM NDSB-211(in drop only), VAPOR DIFFUSION, HANGING DROP, temperature 295K