3E86

High resolution Crystal Structure of the open NaK channel pore

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	I 4
Unit cell lengths	68.030, 68.030, 89.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.010 - 1.600
Rwork	0.212
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	25448

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	100mM Glycine buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 1mM CaCl2, pH 9.5, vapor diffusion, sitting drop, temperature 293K, VAPOR DIFFUSION, SITTING DROP