3E7Q
The crystal structure of the putative transcriptional regulator from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794, 0.9796 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 160.613, 53.706, 57.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.320 - 2.200 |
| R-factor | 0.21674 |
| Rwork | 0.214 |
| R-free | 0.26551 |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.771 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.320 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.700 | 0.618 |
| Number of reflections | 23472 | |
| <I/σ(I)> | 35 | 1.2 |
| Completeness [%] | 1.0 | 0.767 |
| Redundancy | 8.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 20% PEG3350, 0.2M MgNitrate, NDSB 256, 0.3M, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
