3E79
Structure determination of the cancer-associated Mycoplasma hyorhinis protein Mh-p37
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.107, 69.149, 60.297 |
| Unit cell angles | 90.00, 106.81, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.189 |
| R-free | 0.24400 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.021 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 29985 | |
| <I/σ(I)> | 10.8 | 3.7 |
| Completeness [%] | 92.2 | 87 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch | 3 | 298 | 0.1 M Ammonium Bromide, O.1 M Citric Acid, 40% PEG 4000, 9% N-decyl-beta-D-maltoside detergent. Crystal soaked in final concentration 0.7mM 5-amino-2,4,6-triiodoisophthalic acid, pH 3.0, Batch, temperature 298K |
