3E76
Crystal structure of Wild-type GroEL with bound Thallium ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 135.676, 260.954, 287.872 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.390 - 3.940 |
| R-factor | 0.262 |
| Rwork | 0.261 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1XCK |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.919 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.080 |
| High resolution limit [Å] | 3.940 | 3.940 |
| Rmerge | 0.189 | 0.960 |
| Number of reflections | 90513 | |
| <I/σ(I)> | 12.8 | 2.1 |
| Completeness [%] | 98.0 | 88 |
| Redundancy | 8.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
