3E70
Structures and conformations in solution of the Signal Recognition Particle Receptor from the Archaeon Pyrococcus Furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2008-05-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11588 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.122, 53.034, 61.260 |
| Unit cell angles | 90.00, 107.42, 90.00 |
Refinement procedure
| Resolution | 29.690 - 1.970 |
| R-factor | 0.234 |
| Rwork | 0.228 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dmd |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.368 |
| Data reduction software | MOSFLM (IN ELVES) |
| Data scaling software | SCALA (IN ELVES) |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.400 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.049 | 0.447 |
| Number of reflections | 23541 | |
| <I/σ(I)> | 8.7 | 1.7 |
| Completeness [%] | 95.9 | 92.6 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 14-17% PEG 8000 TRIS 100mM pH=8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
