3E6R
Crystal structure of apo-ferritin from Pseudo-nitzschia multiseries
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-02-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9761 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 126.572, 126.572, 170.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.950 - 2.400 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.25200 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.192 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.095 | 0.035 | 0.436 |
| Number of reflections | 52007 | ||
| Completeness [%] | 94.7 | 91 | 96.8 |
| Redundancy | 6.4 | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 1.4 M ammonium sulfate and 0.1 M ammonium acetate pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
