3E6L

Structure of murine INOS oxygenase domain with inhibitor AR-C132283

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-05-10
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	P 61 2 2
Unit cell lengths	213.898, 213.898, 116.148
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.980 - 2.300
Rwork	0.219
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.043	0.216
Number of reflections	68068
Completeness [%]	97.9	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	lithium sulfate, hepes, BOG, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K