3E6G

Crystal structure of XometC, a cystathionine c-lyase-like protein from Xanthomonas oryzae pv.oryzae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-11
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00000
Spacegroup name	P 41
Unit cell lengths	78.351, 78.351, 300.989
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.260 - 2.800
R-factor	0.21957
Rwork	0.216
R-free	0.29549
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i87
RMSD bond length	0.015
RMSD bond angle	1.806
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	44441
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	283	25% PEG3350, 0.2M lithium sulfate monohydrate, 0.1M bis-Tris, pH5.5, sodium thiocyanate, VAPOR DIFFUSION, HANGING DROP, temperature 283K