3E61
Crystal structure of a putative transcriptional repressor of ribose operon from Staphylococcus saprophyticus subsp. saprophyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.146, 106.634, 68.183 |
| Unit cell angles | 90.00, 112.17, 90.00 |
Refinement procedure
| Resolution | 19.749 - 2.000 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25900 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.542 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.749 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.103 | 0.587 |
| Total number of observations | 26663 | |
| Number of reflections | 36116 | |
| <I/σ(I)> | 12.3 | 2.8 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 3M sodium formate, 275mM sodium thiocyanate, pH 7.0, Vapor diffusion, temperature 294K |
